Bibliography:
Comparative analysis of SARS2 PPIs in the context of complement mediated-inflammation.
COVID: the virus was never proven to exist
http://amigo.geneontology.org/amigo/term/GO:0005515
23195 IPR002035 VWF_A
23195 IPR036465 vWFA_dom_sf
Many previously reported affinity capture results were supported with evidence from proximity label-ms experiments that identified proximal interactors Stokalov 2020, Multi-level proteomics reveals host-perturbation strategies of SARS-CoV-2 and SARS-CoV
1186 IPR001807 Cl-channel_volt-gated
1186 IPR002249 Cl_channel-7
1186 IPR014743 Cl-channel_core
R-HSA-9694322, Virion Assembly and Release R-HSA-9694614, Attachment and Entry R-HSA-9694635, Translation of structural proteins R-HSA-9694719, Maturation of protein 3a
https://reactome.org/content/schema/instance/browser/R-HSA-9679509
Insertion of tail-anchored proteins into the endoplasmic reticulum membrane (R-HSA-9609523)
“HMOX1” “CYB5A” “VAMP2” “BAG6” “STX5” “VAPA” “GET4” “UBL4A”
Cell surface interactions at the vessicular wall (R-HSA-202733)
[1] “PROCR” “ITGA6” “TNFRSF10B” “CD99” “TNFRSF10D” “PTPN11” “TNFRSF10A” “CD44”
[9] “CXADR” “SLC7A11” “GLG1” “SLC16A1” “SRC” “SLC16A3” “JAM3” “LYN”
[17] “SLC3A2” “YES1” “ITGB1” “MIF” “PPIA”
ebi.ac.uk/interpro/entry/InterPro/IPR024407/
“Forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. Up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. Induces apoptosis in cell culture. Downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination. The 3a protein is a pro-apoptosis-inducing protein. It localises to the endoplasmic reticulum (ER)-Golgi compartment. SARS-CoV causes apoptosis of infected cells through NLRP3 inflammasome activation, as ORF3a is a potent activator of the signals required for this activation, pro-IL-1beta gene transcription and protein maturation. This protein also promotes the ubiquitination of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) mediated by its interaction with TNF receptor-associated factor 3 (TRAF3). The expression of ORF3a induces NF-kappa B activation and up-regulates fibrinogen secretion with the consequent high cytokine production [4, 5, 6, 7]. Another apoptosis mechanism described for this protein is the activation of the PERK pathway of unfolded protein response (UPR), which causes phosphorylation of eIF2alpha and leads to reduced translation of cellular proteins as well as the activation of pro-apoptotic downstream effectors (i.e ATF4, CHOP) [8].”
SARS-CoV-2 and ORF3a: Nonsynonymous Mutations, Functional Domains, and Viral Pathogenesis
VG: 1489669(ORF3a) | 43740569(ORF3a) | 9714825(ORF3)